| Expression of proliferating cell nuclear antigen in lung cancer. |
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Sun Young Kim, Kyung Joo Lee, Suk Chul Hong, Pyo Sung Han, Jong Jin Lee, Hae Jung Cho, Ju Ock Kim |
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Department of Internal Medicine, Chungnam National University, School of Medicine, Daejeon, Korea |
| 폐암 조직에서의 Proliferating Cell N uclear Antigen (PCN A) 발현에 관한 연구 |
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| Abstract |
Background
Since an important component of carcinogenesis is unregulated growth, many investigators have reported the methods to detect cell proliferation in tissues including PCNA. PCN A is a 36 Kd intranuclear polypeptide and plays a critical role in cell proliferation. Thus progressive dysregulation of proliferation during carcinogenesis can be directly visualized in the paraffin embed. ded tissue using immunohistochemistry for PCNA which has an advantage of simplicity and maintenance of tissue architecture. The heterogeneity of PCNA expression is known to be related with proliferating fraction, histologic grade, DNA ploidy, and susceptibility of anticancer drugs, etc. We analyzed the biologic significance of the expression of PCNA in lung cancer tissues.
Methods
43 lung cancer tissues, which were resected by surgery and were embedded in paraffin, were stained immunohistochemically by one hour MicroProbe System and the results were corelated with cell type, stage, site and survival.
Results
1) Suamous cell type showed high positivity (89%) than in adenocarcinoma (54%).
2) No significant difference related to tumor stage was noticed. 3) No significant difference between primary site and metastatic site was noticed. 4) No significant difference in 12- month survival between positive group and negative group was noticed.
Conclusion
From this study, we concluded that immunohistochemistry for PCNA expression of routinely processed tissue is a simple technique for the assessment of proliferation in non-small cell lung cancer. Whether the labelling index has an independent prognostic value and deserves special attention in pathobiological evaluation in lung cancer remains to be investigated from large series with longer follow-up and to be correlated with multiple biological markers. |
| Key Words:
Lung cancer, Proliferating cell nuclear antigen, Biologic marker |
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