The Effect of Hyaluronan Treatment in Endotoxemic Rats.

Rho, Byung Hak; Kwon, Kun Young; Choi, Won Il

doi:2011.70.5.390

Tuberc Respir Dis > Volume 70(5); 2011 > Article

Original Article

Tuberculosis and Respiratory Diseases 2011;70(5):390-396.
DOI: https://doi.org/10.4046/trd.2011.70.5.390 Published online May 1, 2011.

The Effect of Hyaluronan Treatment in Endotoxemic Rats.

Byung Hak Rho, Kun Young Kwon, Won Il Choi

¹Department of Diagnostic Radiology, Keimyung University School of Medicine, Daegu, Korea.
²Department of Pathology, Keimyung University School of Medicine, Daegu, Korea.
³Department of Internal Medicine, Keimyung University School of Medicine, Daegu, Korea. wichoi@dsmc.or.kr

Abstract

BACKGROUND
Hyaluronan (HA) is an unbranched glycosaminoglycan. It has been proposed that HA acts as a vehicle for cytokines due to the strong negative charge on its surface. We hypothesized that HA would function like a cytokine scavenger and reduce the inflammatory signaling cascade and this would lead to improved survival in rats suffering with endotoxemia. METHODS: Endotoxin (Salmonella, 10 mg/kg) or an equal amount of 0.9% NaCl (NS) was injected into the jugular vein of rats. HA (1,600 kDa, 0.35%) or NS was given at 0.1 mL/kg/h for 3 hours. HA or NS infusion was started at 4 hour after endotoxin injection. The rats were divided into the control and HA groups (n=16 for each group). The mean arterial pressure (MAP) was monitored during HA or normal saline infusion. Survival was assessed every 12 hours for 3 days throughout the experiment. RESULTS: The survival rate (%) of the rats treated with HA was higher (60%) than that of the controls (20%) when HA was infused 4 hours after lipopolysaccharide (LPS) injection. The bronchoalveolar lavage (BAL) fluid of the animals surviving HA or NS infusion 4 hours after LPS showed that the total cell counts and number of neutrophils were significantly (p<0.01) reduced in the HA treated groups compared with that of the controls (total cell count, 9.2x10(4)/mL vs. 61x10(4)/mL; neutrophils, 21x10(4)/mL vs. 0.2x10(4)/mL, respectively). There was no significant MAP difference between the HA or control groups either with or without endotoxin. CONCLUSION: Infusion of hyaluronan (1,600 kDa) reduced the BAL total cell count and the number of neutrophils and it improved the survival rate of the endotoxemic rats.

Key Words: Endotoxemia, Hyaluronic Acid, Survival

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