| STUDIES ON MEDIA FOR CULTIVATION OF TUBERCLE BACILLI 2. Effects of temperature of inspissation and storage Ogawa's egg media and different conditions of cultivation for growth of the tubercle bacilli. |
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YONG HYUN KIM, SUNG CHUNG KIM, HOON HUH, SUNG LIM KIM |
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Department of Clinical Pathology, Soo Do medical, Seoul, Korea |
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| Abstract |
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Heating for coagulation of Ogawa's egg media was started from 55℃ for evaporation of air in inside of the media and the temperature was elevated gradually about 10℃ during fifteen minutes in speed by controlling ther-mostat of electric automatic inspissator. So that, for preparation of egg solid media coagulated at 85℃ it needs about forty-fuve minutes, starting from 55℃. The authors prepared Ogawa's egg media coagulated at different temperatures by using the above processes and compared effects of different temperature of coagulation and storage of Ogawa;s media and different conditions of cultivation for growth of tubercle bacilli. Inoculated media were sealed tightly with rubber caps. 1. Optimal coagulation temperature of Ogawa's egg media for growth of tubercle bacilli was 85℃ and this temperature was more optimal than 90℃ for preparation of Ogawa's media in number of colonies and also in size of colonies. The media coagulated one hour at 90℃ was worse than the above two kinds of media for growth of tubercle bacilli. 2. There were no significant differences for growth of tubercle bacilli between Ogawa's media stored at 10℃ during four months and freshly prepared Ogawa's media. And also Ogaawa's media stored at room temperature during four months from March the July were still useful for cultivation of tubercle bacilli. It seems important for long-time storage of Ogawa's egg media to protect dryness of media as much as low temperature. 3. Tight sealing of inoculated media was better for growth of small number of tubercle bacilli, i.e. from few to several hundred viable units, than incomplete sealing of inoculated media. |
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