Serodiagnosis of Tuberculosis Using Absorbance-difference on Immunoblots

Park, Young Kil; Yoo, Jae Kyung; Kim, Sang Jae

doi:1990.37.2.183

Tuberc Respir Dis > Volume 37(2); 1990 > Article

Original Article

Tuberculosis and Respiratory Diseases 1990;37(2):183-189.
DOI: https://doi.org/10.4046/trd.1990.37.2.183 Published online June 1, 1990.

Serodiagnosis of Tuberculosis Using Absorbance-difference on Immunoblots

Young Kil Park, Jae Kyung Yoo, Sang Jae Kim

Korean lnstituteof Tuberculosis, Korean National Tuberculsosi Association

Immunoblots상의 발색밀도 차이를 이용한 결핵의 혈청학적 진단

박영길, 유재경, 김상재

Abstract

This study was done to develop a serodiagonstic procedure for tuberculosis. The sera from 40 patients with pulmonary tuberculosis who were found by chest X-ray examination, smear and cluture and from 25 healthy persons were reacted with antigen prepared with cell extract of Mycobacterium tuberculosis by immunoblotting. Absorbance 0f the bands visulized by enzyme reaction was measured at 600 nm and analyzed and the results obtained are as follow The specificity of the 24 kD antigen was 100% and the sensitivity, 37.5%. The specificity of the 35 kD antigen was 96% and the sensitivity, 70% Comparative analysis of absorbance of 24 kD, 28 kD, 29 kD and 35 kD antigens permits to diagnose active tuberculosis with the probability of more than 70 9'0. Thus when the absorbance. difference of these antigens is projected, it may be useful for diagnosing patients who are negative on smear and culture examination.