Experimental study on production of Freeze Dried B.C.G. Vaccine in Korea |
Hyun Oh Shin |
Dept. of bacteriology and pathology, The Institute of Tuberculosis, KNTA |
냉동건조(B.C.G.) 생산에 관한 연구(제1보) |
신현오 |
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Abstract |
Author Prepared 30 lots of Freeze dried B.C.G. Vaccine with using Stokes Freeze dryer. The vaccine was dried for 24 hours. in a vacuum of 200 micron at 25 ℃, after few hours initiate freezing at -40 ℃, The number of viable count was determined by the quantitative culture on Löwenstein. Jensen medium.
The survival rate was 37% compared before freeze drying and the viavle rate after 3 months. storage at 5℃ was 81 % and 56% after 6 months. ln regard to the tuberculin conversion rate to schoolchildren, the freeze dried B.C.G. vaccine showed 66% comparing with the wet B.C.G. vaccine which showed 76%. And the size of average induration by the freeze dried vaccine was a little smaller than that by the' wet vaccine, the size of the vaccination lesion by the freeze dried B.C.G. vaccine was smaller then that of wet B.C.G. vaccine. |
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