| Pretreatment of Diltiazem Ameliorates Endotoxin-Induced Acute Lung Injury by Suppression of Neutrophilic Oxidative Stress. |
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Yoo Suk Jang, Young Man Lee, Wook Su Ahn, Sang Chae Lee, Kyung Chan Kim, Dae Sung Hyun |
1Department of Physiology, School of Medicine, Daegu Catholic University, Daegu, Korea. hdsomn@cu.ac.kr
2Department of Chest Surgery, School of Medicine, Daegu Catholic University, Daegu, Korea.
3Department of Internal Medicine, School of Medicine, Daegu Catholic University, Daegu, Korea. |
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| Abstract |
BACKGROUND
Acute respiratory distress syndrome (ARDS) is characterized by severe inflammatory pulmonary edema of unknown pathogenesis. To investigate the pathogenesis of ARDS associated with neutrophilic oxidative stress, the role of phospholipase A2 (PLA2) was evaluated by the inhibition of calcium channel. METHODS: In Sprague-Dawley rats, acute lung injury (ALI) was induced by the instillation of E.coli endotoxin (ETX) into the trachea. At the same time, diltiazem was given 60 min prior to tracheal instillation of ETX. Parameters of ALI such as lung and neutrophil PLA2, lung myeloperoxidase (MPO), BAL neutrophils, protein, surfactant were measured. Production of free radicals from neutrophils was measured also. Morphological studies with light microscope and electron microscope were carried out and electron microscopic cytochemistry for detection of free radicals was performed also. RESULTS: Diltiazem had decreased the ALI parameters effectively in ETX given rats and decreased the production of free radicals from neutrophils and lung tissues. Morphological studies denoted the protective effects of diltiazem. CONCLUSION: Diltiazem, a calcium channel blocker, was effective in amelioration of ALI by the suppression of neutrophilic oxidative stress mediated by PLA2 activation. |
| Key Words:
ARDS, PLA2, Free Radicals, Neutrophils |
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