Presumptive Role of Neutrophilic Oxidative Stress in Oxygen-induced Acute Lung Injury in Rats.

Moon, Yongsuck; Kim, Jihye; Lee, Young Man

doi:2008.65.6.464

Tuberc Respir Dis > Volume 65(6); 2008 > Article

Original Article

Tuberculosis and Respiratory Diseases 2008;65(6):464-470.
DOI: https://doi.org/10.4046/trd.2008.65.6.464 Published online December 1, 2008.

Presumptive Role of Neutrophilic Oxidative Stress in Oxygen-induced Acute Lung Injury in Rats.

Yongsuck Moon, Jihye Kim, Young Man Lee

¹Department of Anatomy, Catholic University of Daegu, School of Medicine, Daegu, Korea.
²Department of Physiology, Catholic University of Daegu, School of Medicine, Daegu, Korea. leeym@cu.ac.kr

Abstract

BACKGROUND
This study examined the role of neutrophilc oxidative stress in an O2-induced acute lung injury (ALI). METHODS: For 48 h, experimental rats were exposed to pure oxygen (normobaric hyperoxia) in a plastic cage. Forty-eight hours after O2 breathing, the rats were sacrificed and the parameters for ALI associated with neutrophilic oxidative stress were assessed. RESULTS: Normobaric pure oxygen induced ALI, which was quite similar to ARDS. The O2-induced neutrophilic oxidative stress was identified by confirming of the increase in lung myeloperoxidase, BAL neutrophils, malondialdehyde (MDA), cytosolic phospholipase A2 (cPLA2) activity in the lung, histological changes and BAL cytospin morphology. CONCLUSION: In part, ALI-caused by oxygen is affected by neutrophils especially by the generation of free radicals.

Key Words: Acute lung injury, O2 toxicity, Neutrophils