Effects of Curcumin and Genistein on Phorbol Ester or Tumor Necrosis Factor-alpha-Induced Mucin Production from Human Airway Epithelial Cells.

Lee, Su Yel; Lee, Hyun Jae; Lee, Jae Woo; Jeon, Byeong Kyou; Kim, Ju Ock; Lee, Choong Jae

doi:2011.70.3.218

Tuberc Respir Dis > Volume 70(3); 2011 > Article

Original Article

Tuberculosis and Respiratory Diseases 2011;70(3):218-223.
DOI: https://doi.org/10.4046/trd.2011.70.3.218 Published online March 1, 2011.

Effects of Curcumin and Genistein on Phorbol Ester or Tumor Necrosis Factor-alpha-Induced Mucin Production from Human Airway Epithelial Cells.

Su Yel Lee, Hyun Jae Lee, Jae Woo Lee, Byeong Kyou Jeon, Ju Ock Kim, Choong Jae Lee

¹Department of Pharmacology, Chungnam National University College of Medicine, Daejeon, Korea. LCJ123@cnu.ac.kr
²LG Life Science, Seoul, Korea.
³Department of Radiologic Technology, Daegu Health College, Daegu, Korea.
⁴Pulmonology Section, Department of Internal Medicine, Chungnam National University Hospital, Chungnam National University College of Medicine, Daejeon, Korea.

Abstract

BACKGROUND
We investigated whether curcumin and genistein affect the MUC5AC mucin production from human airway epithelial cells that is induced by phorbol 12-myristate 13-acetate (PMA) or tumor necrosis factor-alpha (TNF-alpha). METHODS: Confluent NCI-H292 cells were pretreated with each agent for 30 min and then stimulated with PMA or TNF-alpha for 24 hours. MUC5AC mucin production was measured by an ELISA. RESULTS: (1) Curcumin dose-dependently inhibited the production of MUC5AC mucin that was induced by PMA or TNF-alpha; (2) Genistein inhibited PMA-induced MUC5AC mucin production. However, it did not decrease TNF-alpha-induced MUC5AC mucin production. CONCLUSION: These results suggest that curcumin and genistein inhibit the production of airway mucin induced by PMA.

Key Words: Mucin 5AC, Curcumin, Genistein