Mutations of p53 tumor suppressor gene in human lung cancer cell lines.

Hong, Weon Seon; Hong, Seok Il; Lee, Dong Soon; Son, Young Sook; Lee, Choon Taek

doi:1993.40.6.653

Tuberc Respir Dis > Volume 40(6); 1993 > Article

Original Article

Tuberculosis and Respiratory Diseases 1993;40(6):653-658.
DOI: https://doi.org/10.4046/trd.1993.40.6.653 Published online December 1, 1993.

Mutations of p53 tumor suppressor gene in human lung cancer cell lines.

Weon Seon Hong¹, Seok Il Hong¹, Dong Soon Lee², Young Sook Son³, Choon Taek Lee¹

¹Department of Internal Medicine, Korea Cancer Center Hospital, Seoul, Korea
²Department of Clinical Pathology, Korea Cancer Center Hospital, Seoul, Korea
³Medical Research Divison, Korea Cancer Center Hospital, Seoul, Korea

Abstract

Background
Recent advancement of molecular genetics has revealed that malignant transformation of a cell may be a complex multistep process and this process is grouped, in general, into two distinct categories, activation of protooncogenes and inactivation of tumor suppressor genes. This study was focused on the mutation of p53 tumor suppressor gene, because p53 gene mutation is now generally accepted to be one of the most frequent genetic changes in a variety of human cancers Although lung cancer is one of the common cancers in Korea , the genetic change in the carcinogenesis process is not yet known c1 early. To investigate the role of p53 gene mutation in lung cancer, we examined the mutations of exon 4-8 of the p53 gene in humna lung cancer cell lines, because most of the mutations of p53 gene have been reported to develop in exon 4-8.
Methods
Genomic DNA was obtained by the digestion of proteinase K and the extraction by phenol. chloroform.ethanol method from two human pulmonary adenocarcinoma cell lines, PC-9 and PC-14 , and one human small cell lung cancer cell line, H69. To detect the mutations of exon 4-8 of the p53 gene, polymerase chain reaction single. strand conformation polymorphism (PCR.SSCP) analysis was performed with the DNA extracted from the cells.
Results
The mutation of p53 gene was present in all three cell lines tested. In PC-9, PC-14 and H69, the altered mobility was detected in exon 7, 7 and 5, respectively.
conclusion
These results suggest that p53 gene l11 mutation plays an important role in certain steps of the carcinogenesis of human non. small cell and small cell lung cancer

Key Words: Human lung cancer cell lines, p53 gene mutation, PCR-SSCP

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