Tuberc Respir Dis > Volume 45(4); 1998 > Article
Tuberculosis and Respiratory Diseases 1998;45(4):697-704.
DOI: https://doi.org/10.4046/trd.1998.45.4.697    Published online August 1, 1998.
Eotaxin mRNA Expression in Bronchial Mucosa of Patients with Asthma.
Kwang Ho In, Jae Yun Cho, Sae Yong Kang, Sang Youb Lee, Jae Jeong Shim, Kyung Ho Kang, Se Hwa Yoo, Young Soon Na, Han Gyum Kim
1Department of Internal Medicine, College of Medicine, Korea University, Seoul, Korea.
2Department of Pathology, College of Medicine, Korea University, Seoul, Korea.
Abstract
BACKGROUND
Asthma is a chronic inflammatory disease of the airways characterized by a marked infiltration of ecsinophils in the bronchial mucosa. Asthmatic bronchial muosa produces many factors described as king chernotaetic for inflammatory cells. IL-5, RANTES, and MCP-1 alpha are the chemotactic factors for eosinophils, but their roles are controversiaL Recently eotaxin that is a potent eosinophil chernoattracttnt cytokine was detected in a guinea-pig model of allergic airway inflammation, and human eotaxin was cloned. Eotaxin is a specific chemoattractant for eosinophils, but its role in asthma is not confirmed. We examined the in vivo expression of a,taxin in bronchi of asthmatic patients. METHODS: 11 asthmatics and 2 normal controls were enrolled. All subjects were underwent brcnchcscopy with bronchial biopsies in 2nd or 3rd carina. RNA extraction from biopsy samples was done by acid-guanidium method. Semi-quantitaive RT-PCR was done for evaluation of eotaxin mRNA expression. The extent of eosinophil infiltrartion was evaluated by counting the eosinophils in submucosa in HPF of microscope. RESULTS: Eotaxin mRNA expressed in symptomatic, uncontrolled asthma. Steroid inhibited expression of eotaxin mRNA in asthma. Expression of eotaxin mRNA correlated with eosinohil infiltration in bronchial tissues. CONCLUISON: Expression of eotaxin mRNA increases in uncontrolled asthma and eotaxin is involved in the recruitment of eosinophils.
Key Words: Eotaxin, Asthma, Bronchial mucosa


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