Loss of Heterozygosity at 3p in Korean Non-Small Cell Lung Cancer.

Lee, Choon Taek; Kim, Mi Hee; Park, Kyung Ho; Park, Jong Ho; Baek, Hee Jong; Zo, Jae Ill; Kim, Jin Kyoo; Kim, Chang Min

doi:1998.45.5.975

Tuberc Respir Dis > Volume 45(5); 1998 > Article

Original Article

Tuberculosis and Respiratory Diseases 1998;45(5):975-983.
DOI: https://doi.org/10.4046/trd.1998.45.5.975 Published online October 1, 1998.

Loss of Heterozygosity at 3p in Korean Non-Small Cell Lung Cancer.

Choon Taek Lee, Mi Hee Kim, Kyung Ho Park, Jong Ho Park, Hee Jong Baek, Jae Ill Zo, Jin Kyoo Kim, Chang Min Kim

¹Department of Medicine, Seoul National University College of Medicine, Seoul, Korea.
²Laboratory of Molecular Oncology, Korea Cancer Center Hospital, Seoul, Korea.
³Department of Thoracic Surgery, Korea Cancer Center Hospital, Seoul, Korea.
⁴Department of Microbiology, Changwon National University, Changwon, Korea.

Abstract

PURPOSE
Frequent deletion of 3p in numerous cancer including lung cancer suggests the presence of tumor suppressor gene. 3p has been analysed by RFLP and PCR-LOH of microsatellite locus. In this study, we observed the deletion of 3p in Korean NSCLC by PCR-LOH of 4 microsatellite loci and investigated the clinical significance. Method : 62 surgically rejected NSCLC DNA and normal lung DNA have been analysed by PCR-LOH at three dinucleotide[D3S1228 (3p14.1-14.3), D3S1067 (3p14.3-21.1), D3S1029 (3p21.1-21.3)] and one tetranucleotide[D3S1537 (Sp 22-24.2)] repeat microsatellite loci. RESULTS: Among 59 informative cases, 3p deletion by PCR-LOH at four microsatellite loci was found in 31 patients (52.5%). 3p deletion were found in 55% of squamous cell lung cancer and 47% of adenocarcinoma patients. No significant difference has been found in clinical parameters such as staging, smoking and survival according to the status of 3p deletion. CONCLUSION: Deletions in 3p have played an important role in Korean NSCLC though no clinical significance was detected.

Key Words: 3p deletion, Non-small cell lung cancer(NSCLC), Microsatellite, PCR-LOH