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Tuberc Respir Dis > Volume 63(2); 2007 > Article
Tuberculosis and Respiratory Diseases 2007;63(2):145-153.
DOI:    Published online August 1, 2007.
IL-1Ra Elaboration by Colchicine Stimulation in Normal Human Bronchial Epithelial Cells.
Jae Hyung Lee, , Sang Heon Kim, , Tae Hyung Kim, , Jang Won Sohn, , Ho Joo Yoon, , Dong Ho Shin, , Sung Soo Park,
1Department of Internal Medicine, Eulji General Hospital, Eulji University School of Medicine, Seoul, Korea.
2Department of Internal Medicine, College of Medicine, Hanyang University, Seoul, Korea.
Asthma is a syndrome that is characterized by a variable degree of airflow obstruction, bronchial hyperresponsiveness, and airway inflammation. Colchicine is an inexpensive and safe medication with unique anti- inflammatory properties. IL-1Ra (Interleukin-1 receptor antagonist) mediates the anti-inflammatory effect in human inflammatory diseases, including asthma. This study examined whether IL-1Ra mediates the anti-inflammatory effect of colchicine in normal human bronchial epithelial cells (NHBE), RAW 264.7 cells (murine macrophage cell line), and a mouse lung. METHODS: NHBE, RAW 264.7 cells and BALB/c mice were stimulated with colchicine, and the increase in the IL-1Ra level was estimated by ELISA, Western analysis and RT-PCR analysis. RESULTS: Colchicine stimulated NHBE and RAW 264.7 cells to release IL-1Ra into the supernatant in a dose-and time-dependent manner. The major isoform of IL-1Ra in NHBE and RAW 264.7 cells is type I icIL-1Ra, and sIL-1Ra, respectively. IL-1Ra up-regulation was blocked by PD98059, a specific inhibitor in MAPK pathways. Colchicine also stimulated the secretion of IL-1Ra into the bronchoalveolar lavage (BAL) fluid of BALB/c mouse. CONCLUSION: Colchicine stimulates an increase in the IL-1Ra level both in vivo and in vitro, and might have an anti-inflammatory effect.
Key Words: Colchicine, Asthma, IL-1Ra, MAPK
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