Expression of Estrogen and Progesterone Receptors in Non- small-cell Lung Cancer Tissue Using Tissue Microarray Method.

Han, Hye Seung; Kim, Min Ji; Cho, Jae Hwa; Yoon, Yong Han; Kwak, Seung Min; Lee, Hong Lyeol; Kim, Kwang Ho; Ryu, Jeong Seon

doi:2005.58.1.54

Tuberc Respir Dis > Volume 58(1); 2005 > Article

Original Article

Tuberculosis and Respiratory Diseases 2005;58(1):54-58.
DOI: https://doi.org/10.4046/trd.2005.58.1.54 Published online January 1, 2005.

Expression of Estrogen and Progesterone Receptors in Non- small-cell Lung Cancer Tissue Using Tissue Microarray Method.

Hye Seung Han, Min Ji Kim, Jae Hwa Cho, Yong Han Yoon, Seung Min Kwak, Hong Lyeol Lee, Kwang Ho Kim, Jeong Seon Ryu

¹Department of Pathology, College of Medicine, Konkuk University, Seoul, Korea.
²Department of Internal Medicine, College of Medicine, Inha University, Incheon, Korea. jsryu@inha.ac.kr
³Department of Chest Surgery, College of Medicine, Inha University, Incheon, Korea.

Abstract

BACKGROUND
To evaluate the role of estrogen and progesterone in the carcinogenesis of NSCLC, IHC studies for the expression of the receptors of estrogen and pro?gesterone have been performed with inconsistent results. Recently the TMA method has been developed and has become recognized as a useful and rapid method for extensively analysing molecular markers at the gene and protein level. We have investigated their expressions in the tissue from NSCLC using the microarray method. METHODS: The TMA construction was made with 70 formalin- fixed, paraffin-embedded tissues of NSCLC. After heat-induced epitope retrieval, IHC staining on pri?mary tissues of NSCLC was performed with the monoclonal antibodies, ER1D5 and PR1A6. RESULTS: Our sample of 70 consisted of 74% men and 26% women. Of the patients, 49% were current smokers, 27% were non-smokers and 24% were former smokers. By histologic classification, 34 patients had squamous cell carcinoma, 24 had adenocarcinoma, 9 had adenosquamous cell carcinoma, and 3 had other carcinomas. No cancer cells were immunostained with these monoclonal antibodies in any primary tissues of NSCLC. CONCLUSIONS: No expression of neither of the two receptors was found in any of the lung cancer tissues. This su?ggests that adequate genetic variants for IHC staining need to be developed for NSCLC.

Key Words: Lung Cancer, Receptor, Estrogen, Progesterone