| Usefulness of Automated PCR Test for the Detection of Mycobacterium Tuberculosis in Fresh Biopsy Tissues. |
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Woo Soon Choi, So Young Shin, Jong Ok Kim, Myung Sook Kim, Hye Kyung Lee |
1Department of Laboratory Medicine, College of Medicine, The Catholic University, Daejeon, Korea.
2Department of Pathology, College of Medicine, The Catholic University, Daejeon, Korea. apw01@hanmail.net
3Department of Internal Medicine, College of Medicine, The Catholic University, Daejeon, Korea. |
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| Abstract |
BACKGROUND
Although there have been several studies regarding the clinical value of an automated TB-PCR study using sputum, bronchial washing, and other body fluid samples for the detection of pulmonary tuberculosis, there are only a few reports on the use of fresh tissue samples. MATERIALS AND METHODS: The acid-fast bacilli stain(AFB), tuberculosis culture, automated TB-PCR study, and histopathology examination were performed in 42 fresh tissue samples. RESULTS: Among the 42 cases, 18 cases were diagnosed with tuberculosis based on the clinical findings. Sixteen of the 18 cases were TB-PCR positive and of these 16 cases, only 2 cases were positive in the AFB stain or culture study. However, all 18 cases showed the histopathology findings of chronic granulomatous inflammation that was compatible with tuberculosis. Based on the clinical findings, the sensitivity, specificity, positive predictability, and negative predictability of the automated TB-PCR study were 88.9%, 100%, 100%, and 92.3% respectively. CONCLUSION: An automated TB-PCR assay is an important diagnostic tool for diagnosing tuberculosis in fresh tissue samples. |
| Key Words:
Fresh tissue sample, Automated tuberculosis polymerase chain reaction(TB-PCR) |
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