| Detection of Clarithromycin-resistant Strains from Clinical Isolates of Mycobacterium abscessus. |
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Seung Heon Lee, Young Kil Park, Sung Weon Ryo, Myung Sup Shim, Woo Jin Lew, Hee Jin Kim |
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Korean Institute of Tuberculosis, Seoul, Korea. hatchingbird@yahoo.co.kr |
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| Abstract |
BACKGROUND
Mycobacterium abscessus is the most pathogenic and drug-resistant rapid-growing mycobacterium. Clarithromycin or azithromycin are the only regular oral antimycobacterial agents that have an effect on M. abscessus. We tried to detect the clarithromycin-resistant strains from the clinical isolates of M. abscessus. METHODS: We tried to isolate the clarithromycin-resistant strains from 220 clinical isolates of M. abscessus by performing using reverse hybridization assay (RHA) and the broth microdilution test (BMT). RESULTS: Seven resistant strains (3.2%) from all the tested clinical isolates were detected by BMT. Three of these resistant strains were also detected by RHA and it was confirmed that they had point mutants. CONCLUSION: These results showed that clarithromycin resistance in M. abscessus clinical isolates is related to a point mutation and other unknown mechanisms. |
| Key Words:
Mycobacterium abscessus, Reverse hybridization assay, Broth microdilution test, 23S rRNA, Clarithromycin |
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